8-Hydroxyguanosine is an oxidized nucleoside and a marker of RNA oxidative damage.1 Levels of 8-hydroxyguanosine increase in HeLa cells in a concentration-dependent manner when cultured with hydrogen peroxide. It has been detected in neurons in postmortem brain from patients with Alzheimer's or Parkinson's disease.2,3
1.Wu, J., and Li, Z.Human polynucleotide phosphorylase reduces oxidative RNA damage and protects HeLa cell against oxidative stressBiochem. Biophys. Res. Commun.372(2)288-292(2008) 2.Nunomura, A., Perry, G., Pappolla, M.A., et al.RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's diseaseJ. Neurosci.19(6)1959-1964(1999) 3.Zhang, J., Perry, G., Smith, M.A., et al.Parkinson's disease is associated with oxidative damage to cytoplasmic DNA and RNA in substantia nigra neuronsAm. J. Pathol.154(5)1423-1429(1999)