7-Deaza-GTP是一种单体型原料，可用于核酸合成。
Sample solution is provided at 25 µL, 10mM.
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7-Deaza-GTP is a monomeric raw material that can be used for nucleic acid synthesis [1]. 7-Deaza-GTP can improve the amplification of GC-rich DNA sequences in polymerase chain reaction (PCR)[2]. 7-Deaza-GTP can enhance amplification of long PCR products and of a random sequence DNA library [3]. 7-Deaza-GTP can be used during preparative-scale enzymatic synthesis of RNAs more than 30 nucleotides long and to abolish abrupt termination [4].
References:
[1] Kuzmine I, Gottlieb P A, Martin C T. Structure in nascent RNA leads to termination of slippage transcription by T7 RNA polymerase[J]. Nucleic acids research, 2001, 29(12): 2601-2606.
[2] McConlogue L, Brow M A, Innis M A. Structure-independent DNA amplification by PCR using 7-deaza-2'-deoxyguanosine[J]. Nucleic acids research, 1988, 16(20): 9869.
[3] Musso M, Bocciardi R, Parodi S, et al. Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences[J]. The Journal of Molecular Diagnostics, 2006, 8(5): 544-550.
[4] Kuzmine I, Gottlieb P A, Martin C T. Binding of the priming nucleotide in the initiation of transcription by T7 RNA polymerase[J]. Journal of Biological Chemistry, 2003, 278(5): 2819-2823.
7-Deaza-GTP是一种单体型原料，可用于核酸合成[1]。7-Deaza-GTP能够改善聚合酶链式反应（PCR）中富含GC的DNA序列的扩增[2]。7-Deaza-GTP可增强长PCR产物及随机序列DNA文库的扩增[3]。7-Deaza-GTP可用于长度超过30个核苷酸的RNA在制备规模上的酶促合成，并能消除突发的合成终止[4]。
| Cas No. | SDF | Download SDF | |
| 分子式 | C11H17N4O14P3 (free acid) | 分子量 | 522.1 (free acid) |
| 溶解度 | 储存条件 | Store at -20°C or below | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9153 mL | 9.5767 mL | 19.1534 mL |
| 5 mM | 383.1 μL | 1.9153 mL | 3.8307 mL |
| 10 mM | 191.5 μL | 957.7 μL | 1.9153 mL |
Quality Control & SDS
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Purity: >98.00%
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