7-Deaza-GTP is a monomeric raw material that can be used for nucleic acid synthesis [1]. 7-Deaza-GTP can improve the amplification of GC-rich DNA sequences in polymerase chain reaction (PCR)[2]. 7-Deaza-GTP can enhance amplification of long PCR products and of a random sequence DNA library [3]. 7-Deaza-GTP can be used during preparative-scale enzymatic synthesis of RNAs more than 30 nucleotides long and to abolish abrupt termination [4].
References:
[1] Kuzmine I, Gottlieb P A, Martin C T. Structure in nascent RNA leads to termination of slippage transcription by T7 RNA polymerase[J]. Nucleic acids research, 2001, 29(12): 2601-2606.
[2] McConlogue L, Brow M A, Innis M A. Structure-independent DNA amplification by PCR using 7-deaza-2'-deoxyguanosine[J]. Nucleic acids research, 1988, 16(20): 9869.
[3] Musso M, Bocciardi R, Parodi S, et al. Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences[J]. The Journal of Molecular Diagnostics, 2006, 8(5): 544-550.
[4] Kuzmine I, Gottlieb P A, Martin C T. Binding of the priming nucleotide in the initiation of transcription by T7 RNA polymerase[J]. Journal of Biological Chemistry, 2003, 278(5): 2819-2823.
7-Deaza-GTP是一种单体型原料，可用于核酸合成[1]。7-Deaza-GTP能够改善聚合酶链式反应（PCR）中富含GC的DNA序列的扩增[2]。7-Deaza-GTP可增强长PCR产物及随机序列DNA文库的扩增[3]。7-Deaza-GTP可用于长度超过30个核苷酸的RNA在制备规模上的酶促合成，并能消除突发的合成终止[4]。