5-TAMRA-SE (5-TAMRA-NHS ester)

5-TAMRA-SE (5-TAMRA-NHS ester) (5-carboxytetramethylrhodamine succinimidyl ester) is a bright orange-red fluorescent dye with maximum excitation/emission wavelengths of approximately 555/580nm^[1]. The NHS ester group of 5-TAMRA-SE can react specifically with primary amine groups and is widely used to label biomolecules such as proteins, peptides, and antibodies under weak alkaline conditions^{[2, 3]}. 5-TAMRA-SE has high fluorescence intensity and good photostability, making it suitable for applications in fluorescence microscopy and flow cytometry^{[4, 5]}. 5-TAMRA-SE is a cell membrane permeable dye that can enter the bacterial cytoplasm and label metabolically active living bacteria^[6].

References:
[1] Karabadzhak A G, An M, Yao L, et al. pHLIP-FIRE, a cell insertion-triggered fluorescent probe for imaging tumors demonstrates targeted cargo delivery in vivo[J]. ACS chemical biology, 2014, 9(11): 2545-2553.
[2] Brunner A, Minamitake Y, Göpferich A. Labelling peptides with fluorescent probes for incorporation into degradable polymers[J]. European journal of pharmaceutics and biopharmaceutics, 1998, 45(3): 265-273.
[3] Patil U S, Qu H, Caruntu D, et al. Labeling primary amine groups in peptides and proteins with N-hydroxysuccinimidyl ester modified Fe3O4@ SiO2 nanoparticles containing cleavable disulfide-bond linkers[J]. Bioconjugate chemistry, 2013, 24(9): 1562-1569.
[4] Archipowa N, Wittmann L, Köckenberger J, et al. Characterization of fluorescent dyes frequently used for bioimaging: Photophysics and photocatalytical reactions with proteins[J]. The Journal of Physical Chemistry B, 2023, 127(44): 9532-9542.
[5] Zeng Q H, Zhang X W, Xu K P, et al. Application of fluorescently labeled tracer technique for detection of natural active macromolecules in Chinese medicine[J]. Drug Metabolism Reviews, 2014, 46(1): 57-71.
[6] Whelan M V X, Ardill L, Koide K, et al. Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni[J]. Scientific Reports, 2019, 9(1): 18216.

5-TAMRA-SE (5-TAMRA-NHS ester)（5-羧基四甲基罗丹明琥珀酰亚胺酯）是一种明亮的橙红色荧光染料，最大激发/发射波长约为555/580nm^[1]。5-TAMRA-SE的NHS酯基团能够特异性地与伯胺基团反应，在弱碱性条件下广泛用于标记蛋白质、多肽和抗体等生物分子^{[2, 3]}。5-TAMRA-SE荧光强度高、光稳定性好，适用于荧光显微镜、流式细胞术应用^{[4, 5]}。5-TAMRA-SE是一种细胞膜渗透性染料，可进入细菌细胞质，标记代谢活跃的活细菌^[6]。