5-Me-CTP,100mM Sodium Solution is a modified nucleoside triphosphate that is commonly used to replace naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis, increasing mRNA stability and protein expression while reducing the interaction between innate immune receptors and immune cells[1]. 5-Me-CTP is the only naturally occurring modified base in animal DNA and is found in mRNA, rRNA, and tRNA of various representative organisms[2]. Methylation of the fifth C atom of 5-Me-CTP is a reversible epigenetic modification that can regulate the binding of transcription factors or induce the binding of specific 5-mC binding proteins when present near gene regulatory regions, leading to the recruitment of co-repressor complexes to methylated target promoters[3]. 5-Me-CTP modification of RNA affects the fate of RNA molecules, including promoting mRNA stability, splicing, and nucleocytoplasmic transport; viral protein expression; DNA damage repair; cell tolerance, proliferation, and migration; and stem cell development, differentiation, and reprogramming[3]. The distribution of 5-Me-CTP varies among cell types, and 5-Me-CTP modifications at specific locations in mRNA exhibit different regulatory activities[4].
References:
[1] Steinle H, Ionescu T M, Schenk S, et al. Incorporation of synthetic mRNA in injectable chitosan-alginate hybrid hydrogels for local and sustained expression of exogenous proteins in cells[J]. International Journal of Molecular Sciences, 2018, 19(5): 1313.
[2] Riggs A D, Jones P A. 5-methylcytosine, gene regulation, and cancer[J]. Advances in cancer research, 1983, 40: 1-30.
[3] Breiling A, Lyko F. Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond[J]. Epigenetics & chromatin, 2015, 8: 1-9.
[4] Song H, Zhang J, Liu B, et al. Biological roles of RNA m5C modification and its implications in Cancer immunotherapy[J]. Biomarker research, 2022, 10(1): 15.
5-Me-CTP,100mM Sodium Solution是一种修饰的三磷酸核苷，通常用于在体外mRNA合成过程中替代天然存在的三磷酸胞苷（CTP），增加mRNA的稳定性和蛋白表达，同时减少天然免疫受体与免疫细胞的相互作用[1]。5-Me-CTP是动物DNA中唯一天然存在的修饰碱基，在各种代表性生物的mRNA、rRNA和tRNA中均有发现[2]。5-Me-CTP第五位C原子甲基化是一种可逆的表观遗传修饰，当存在于基因调控区域附近时能够调节转录因子的结合或诱导特异性5-mC结合蛋白的结合，从而导致共阻遏物复合物募集到甲基化靶启动子[3]。5-Me-CTP修饰RNA后会影响RNA分子的命运，包括促进mRNA稳定性、剪接和核质运输；病毒蛋白表达；DNA损伤修复；细胞耐受性、增殖和迁移；干细胞的发育、分化和重编程[3]。5-Me-CTP的分布因细胞类型而异，在mRNA的特定位置上的5-Me-CTP修饰表现出不同的调控活性[4]。