5-FAM (5-Carboxyfluorescein) is a green fluorescent probe in the form of a single isomer, widely used for labeling peptides, proteins, and nucleotides, with Ex/Em of 490nm/520nm[1]. The single-isomer form of 5-FAM ensures consistent and predictable fluorescence signals, making it suitable for applications such as real-time quantitative PCR (qPCR), fluorescence in situ hybridization (FISH), and fluorescence resonance energy transfer (FRET)[2,3,4].
References:
[1] TOKMINA-ROSZYK M, TOKMINA-ROSZYK D, FIELDS G B. The synthesis and application of Fmoc-Lys (5-Fam) building blocks[J]. Peptide Science, 2013, 100(4): 347-355.
[2] LEE J, SAMSON A A, SONG J M. Peptide substrate-based inkjet printing high-throughput MMP-9 anticancer assay using fluorescence resonance energy transfer (FRET)[J]. Sensors and Actuators B: Chemical, 2018, 256: 1093-1099.
[3] TIMKEN M D, SWANGO K L, ORREGO C, et al. A duplex real-time qPCR assay for the quantification of human nuclear and mitochondrial DNA in forensic samples: implications for quantifying DNA in degraded samples[J]. Journal of Forensic Sciences, 2005, 50(5): JFS2004423-17.
[4] TORIMURA M, KURATA S, YAMADA K, et al. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base[J]. Analytical Sciences, 2001, 17(1): 155-160.
5-FAM (5-Carboxyfluorescein)是一种单一异构体形式的绿色荧光探针，被广泛用于标记肽、蛋白质和核苷酸，Ex/Em为490nm/520nm[1]。5-FAM单一异构体形式确保了荧光信号的一致性和可预测性，适用于实时定量PCR（qPCR）、荧光原位杂交（FISH）和荧光共振能量转移（FRET）等领域[2,3,4]。