5-Aminofluorescein is a fluorescent compound and an inhibitor of the mRNA N6-methyladenosine (m6A) demethylase fat mass and obesity-associated (FTO) protein (IC50 = 6.55 ?M).1,2 It displays excitation/emission maxima of 490 and 515 nm, respectively, and has been conjugated to human serum albumin for fluorescence imaging of glioblastomas.1,3
1.Al-Natour, M.A., Yousif, M.D., Cavanagh, R., et al.Facile dye-initiated polymerization of lactide-glycolide generates highly fluorescent poly(lactic-co-glycolic acid) for enhanced characterization of cellular deliveryACS Macro Lett.9(3)431-437(2020) 2.Wang, T., Hong, T., Huang, Y., et al.Fluorescein derivatives as bifunctional molecules for the simultaneous inhibiting and labeling of FTO proteinJ. Am. Chem. Soc.137(43)13736-13739(2015) 3.Ding, R., Frei, E., Fardanesh, M., et al.Pharmacokinetics of 5-aminofluorescein-albumin, a novel fluorescence marker of brain tumors during surgeryJ. Clin. Pharmacol.51(5)672-678(2011)