4-Thiouridine(4sU) is a ribonucleoside analogue that can be used for RNA analysis and (m)RNA labeling[1]. 4-Thiouridine is a sulfur-containing uridine derivative in which the oxygen atom at the 4-position of the uridine ring is replaced with a sulfur atom. This modification is naturally found in certain RNA molecules, particularly in bacterial transfer RNA (tRNA), where it plays a role in stabilizing RNA structure and enhancing its function[2-3].
In laboratory applications, 4-thiouridine serves as a labeling tool to study RNA synthesis, metabolism, and localization[4-5]. Upon UV irradiation, s4U-labeled RNA can form covalent cross-links with adjacent molecules, such as proteins, which facilitates the analysis of RNA interactions and dynamics within cells[6].
4-Thiouridine can interfere with nucleolar integrity and trigger a nucleolar stress response. 4-Thiouridine triggers translocation of NPM1, stabilization of p53, and inhibition of proliferation [7].
References:
[1]. Dölken L, Ruzsics Z, et,al. High-resolution gene expression profiling for simultaneous kinetic parameter analysis of RNA synthesis and decay. RNA. 2008 Sep;14(9):1959-72. doi: 10.1261/rna.1136108. Epub 2008 Jul 24. PMID: 18658122; PMCID: PMC2525961.
[2]. Hafner M, Landthaler M, et,al. Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP. Cell. 2010 Apr 2;141(1):129-41. doi: 10.1016/j.cell.2010.03.009. PMID: 20371350; PMCID: PMC2861495.
[3]. Windhager L, Bonfert T, et,al. Ultrashort and progressive 4sU-tagging reveals key characteristics of RNA processing at nucleotide resolution. Genome Res. 2012 Oct;22(10):2031-42. doi: 10.1101/gr.131847.111. Epub 2012 Apr 26. PMID: 22539649; PMCID: PMC3460197.
[4]. Rädle B, Rutkowski AJ, et,al. Metabolic labeling of newly transcribed RNA for high resolution gene expression profiling of RNA synthesis, processing and decay in cell culture. J Vis Exp. 2013 Aug 8;(78):50195. doi: 10.3791/50195. PMID: 23963265; PMCID: PMC3854562.
[5]. Schwanhäusser B, Busse D, et,al. Global quantification of mammalian gene expression control. Nature. 2011 May 19;473(7347):337-42. doi: 10.1038/nature10098. Erratum in: Nature. 2013 Mar 07;495(7439):126-7. doi: 10.1038/nature11848. PMID: 21593866.
[6]. Sun W, Chen W. Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay. Methods Mol Biol. 2018;1720:25-34. doi: 10.1007/978-1-4939-7540-2_3. PMID: 29236249.
[7]. Burger K, Mühl B, et,al. 4-thiouridine inhibits rRNA synthesis and causes a nucleolar stress response. RNA Biol. 2013 Oct;10(10):1623-30. doi: 10.4161/rna.26214. Epub 2013 Sep 4. PMID: 24025460; PMCID: PMC3866244.
4-Thiouridine 4-硫脲(4sU)是核糖核苷类似物，可用于RNA分析和(m)RNA标记[1]。4-Thiouridine是一种含硫的尿嘧啶衍生物，其中尿嘧啶环上4位的氧原子被硫原子取代。这种修饰在某些RNA分子中自然存在，特别是在细菌转移RNA (tRNA)中，它在稳定RNA结构和增强其功能方面发挥作用[2-3]。
在实验室应用中，4-Thiouridine可作为研究RNA合成、代谢和定位的标记工具[4-5]。在紫外线照射下，4-Thiouridine标记的RNA可以与邻近的分子(如蛋白质)形成共价交联，这有助于分析RNA在细胞内的相互作用和动力学[6]。
4-Thiouridine可以干扰核仁完整性并引发核仁应激反应。4-Thiouridine触发NPM1易位，稳定p53，抑制增殖[7]。