4-Methylumbelliferyl Oleate is a fluorogenic substrate that releases 4-methylumbelliferone (4-MU; Ex/Em: 320/450nm) upon cleavage by lipases, features a pH-dependent excitation wavelength (330nm at pH 4.6, 370nm at pH 7.4, and 385nm at pH 10.4), and is widely used for detecting acid and alkaline lipase activity in biological samples[1,2,3]. 4-Methylumbelliferyl Oleate offers high sensitivity, produces a blue fluorescent signal upon hydrolysis, and is suitable for lipase kinetic studies and the screening of lipase inhibitors (e.g., pancreatic lipase)[4,5,6].
Reference:
[1] ZHANG J, KANG M J, KIM M J, et al. Pancreatic lipase inhibitory activity of taraxacum officinale in vitro and in vivo[J]. Nutrition Research and Practice, 2008, 2(4): 200-203.
[2] KOSTER J F, VAANDRAGER H, VAN BERKEL T J C. Study of the hydrolysis of 4-methylumbelliferyl oleate by acid lipase and cholesteryl oleate by acid cholesteryl esterase in human leucocytes, fibroblasts and liver[J]. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism, 1980, 618(1): 98-105.
[3] SHIMURA S, TSUZUKI W, SUZUKI T. Determination of lipase activity in organic solvent using fluorescent substrate and its application to the screening of lipase inhibitor[J]. Analytical Sciences, 1991, 7(1): 15-18.
[4] ZHANG J, YANG Y, QIAN X K, et al. Design, Synthesis, and Structure-Activity Relationship Study of Pyrazolones as Potent Inhibitors of Pancreatic Lipase[J]. ChemMedChem, 2021, 16(10): 1600-1604.
[5] NYFELER E, GROGNUX J, WAHLER D, et al. A sensitive and selective high‐throughput screening fluorescence assay for lipases and esterases[J]. Helvetica Chimica Acta, 2003, 86(8): 2919-2927.
[6] SOSNOWSKA D, PODSĘDEK A, KUCHARSKA A Z, et al. Comparison of in vitro anti-lipase and antioxidant activities, and composition of commercial chokeberry juices[J]. European Food Research and Technology, 2016, 242(4): 505-515.
4-Methylumbelliferyl Oleate是一种在被脂肪酶切割后可释放4-甲基伞形酮（4-MU; Ex/Em: 320/450nm）的荧光底物，激发波长依赖于pH值（pH 4.6时为330nm，pH 7.4时为370nm，pH 10.4时为385nm），被广泛应用于生物样本中酸性和碱性脂肪酶活性的检测[1,2,3]。4-Methylumbelliferyl Oleate灵敏度高，水解后产生蓝色荧光信号，适用于脂肪酶动力学研究及脂肪酶抑制剂筛选（如胰腺脂肪酶）等领域[4,5,6]。