4-Methylumbelliferyl Caprylate is substrate that fluoresces upon hydrolysis of the ester bond [1]. 4-Methylumbelliferyl Caprylate is often used as an indicator, which exhibits a relatively high fluorescence intensity at a pH of 6.8. and is used for the identification of Salmonella, with high specificity, sensitivity and usability[2-3]. 4-Methylumbelliferyl Caprylate has been widely used in the development of high-throughput fluorescent esterase activity detection methods for evaluating the risk of polyethylene glycol degradation during the development of biopharmaceuticals[4].
References:
[1] Al-Kady A S, Ahmed E S I, Gaber M, et al. Kinetics of catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2011, 79(5): 1540-1545.
[2] Abdalla S, Vila J, Jimenez de Anta M T. Identification of Salmonella spp. with Rambach agar in conjunction with the 4-methylumbelliferyl caprylate (MUCAP) fluorescence test[J]. British journal of biomedical science, 1994, 51(1): 5-8.
[3] Tang F, Xiong Y, Zhang H, et al. Visual detection technique for efficient screening and isolation of Salmonella based on a novel enrichment assay using chromatography membrane[J]. European Journal of Clinical Microbiology & Infectious Diseases, 2016, 35(3): 353-361.
[4] Bhargava A C, Mains K, Siu A, et al. High-Throughput, Fluorescence-Based Esterase Activity Assay for Assessing Polysorbate Degradation Risk during Biopharmaceutical Development: Bhargava et al[J]. Pharmaceutical Research, 2021, 38(3): 397-413.
4-Methylumbelliferyl Caprylate是一种底物，在酯键水解后会产生荧光[1]。4-Methylumbelliferyl Caprylate常用作指示剂，在pH 6.8时表现出较高的荧光强度，并用于沙门氏菌的鉴定，具有高特异性、高灵敏度和良好的可用性[2-3]。4-Methylumbelliferyl Caprylate已被广泛用于开发高通量荧光酯酶活性检测方法，以评估生物制药开发过程中聚乙二醇降解的风险[4]。