4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG)

目录号: GC30519纯度: >99.50%同义词: 4-甲基伞形酮-Β-D-葡糖苷酸二水合物

4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) 是一种荧光底物 (Λex=362 nm, Λem=445 nm)。

Cas No.: 881005-91-0

规格	价格	库存	数量
25mg	¥360.00	现货	1
50mg	¥576.00	现货	1
100mg	¥922.00	现货	1
10mM (in 1mL DMSO)	¥396.00	现货	1

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187(9):2288-2304 (2024)
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183(7):1867-1883 (2020)
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产品描述 Description

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat with λex=362 nm and λem=445 nm. The half-saturation constants, Km, that are calculated for sewage samples are close to the Km values calculated based on experimental plots presented previously for freshwaters[1].

[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.

实验参考方法 Experimental Reference Method

Kinase experiment:

GLUase activity of E. coli cells is measured. Three millilitres of 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUGlu) solution (55 mg of hydrated MUGlu and 20 mL of Triton X-100 in 50 mL of sterile water) is added to each flask (final concentration:165 mg/L). The incubation temperature is 44°C. One hundred microlitres of 2 M NaOH solution is added to each 2.9-mL aliquot to obtain a pH>10 before the fluorescence measurement[1].

References:

[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.

产品文档 Product Documents

Purity:>99.50%

化学性质Chemical Properties

CAS 号

881005-91-0

同义词

4-甲基伞形酮-Β-D-葡糖苷酸二水合物

SMILES

O[C@H]([C@H]([C@@H]([C@@H](C(O)=O)O1)O)O)[C@@H]1OC2=CC=C(C(O3)=C2)C(C)=CC3=O.O

分子式

C₁₆H₁₈O₁₀

分子量

370.31 g/mol

溶解性

DMSO : 150 mg/mL (405.07 mM);Water : 5.2 mg/mL (14.04 mM)

保存条件

Store at 4°C, protect from light

General tips

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。

Shipping Condition

评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

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4-Methylumbelliferyl-&beta;-D-glucuronide hydrate (MUG)

4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG)