4-Methylumbelliferyl-β-D-Glucopyranoside is a β-D-glucoside that serves as a fluorescent substrate for β-glucosidase, thereby allowing for the detection and analysis of this enzyme's activity[1]. The enzymatic cleavage of 4-Methylumbelliferyl β-D-glucopyranoside results in the release of the highly fluorescent compound 4-Methylumbelliferone. The maximum emission wavelength of 4-MU ranges from 445 to 454nm, while its excitation wavelength varies with pH: at pH 4.6, 7.4, and 10.4, the corresponding excitation wavelengths are approximately 330nm, 370nm, and 385nm, respectively[2].
References:
[1] Van Roey P, Salerno JM. Structure of 4-methylumbelliferyl-beta-D-glucopyranoside. Acta Crystallogr C. 1988 May 15;44 ( Pt 5):865-7.
[2] Mikhaylova M, Wiederschain G, Mikhaylov V, et al. The enzymatic hydrolysis of 6-acylamino-4-methylumbelliferyl-beta-D-glucosides: identification of a novel human acid beta-glucosidase. Biochim Biophys Acta. 1996 Oct 7;1317(1):71-9.
4-Methylumbelliferyl-β-D-Glucopyranoside是一种β-D-葡萄糖苷，可作为β-葡萄糖苷酶的荧光底物，用于检测该酶的活性[1]。4-Methylumbelliferyl-β-D-Glucopyranoside在酶促反应中会释放出高荧光强度的4-Methylumbelliferone，4-Methylumbelliferone最大发射波长为445-454nm，并且其激发波长会随pH值变化，在pH为4.6、7.4和10.4时分别对应330nm、370nm和385nm[2]。