4-Hydroxyphenyl acetate (4-HPA) is a natural antioxidant and protects cells from oxidative stress-induced necrosis. 4-Hydroxyphenyl acetate blocks the increase of cellular ROS induced by oxidative stress, and up-regulates NQO1 and HO-1 genes by stabilizing and inducing the nuclear translocation of NRF2 transcription factor[1].
4-Hydroxyphenyl acetate (5 μM; 24 h) protects retinal pigment epithelial (RPE) cells from oxidative stress-induced cell death[1].
4-Hydroxyphenyl acetate (5 μM; 24 h; ARPE-19 cells) inhibits the increase of ROS in response to oxidative stress and up-regulated the expression of cytoprotective genes including NQO1 and HO-1 genes[1].
Cell Viability Assay[1]
| Cell Line: | ARPE-19 cells |
| Concentration: | 5 μM |
| Incubation Time: | 24 hours |
| Result: | Protected up to 89%, 92%, and 90% of ARPE-19 cells exposed to 100, 200, and 300 μM tBHP, respectively. |
[1]. Hanus J, et, al. 4-Acetoxyphenol Prevents RPE Oxidative Stress-Induced Necrosis by Functioning as an NRF2 Stabilizer. Invest Ophthalmol Vis Sci. 2015 Aug;56(9):5048-59.