3-hydroxy-3-methylglutaryl-Coenzyme A-d3 is intended for use as an internal standard for the quantification of 3-hydroxy-3-methylglutaryl-coenzyme A by GC- or LC-MS. 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) is an intermediate in several metabolic pathways.1,2,3,4 Conversion of HMG-CoA to mevalonate by HMG-CoA reductase is the rate-limiting first step in the cholesterol biosynthetic pathway.3,2 Alternatively, HMG-CoA can be cleaved into acetyl-CoA and the ketone body acetoacetate in mitochondria by HMG-CoA lyase.4,1 HMG-CoA is also an intermediate in the degradation of leucine.4
1.Laffel, L.Ketone bodies: A review of physiology, pathophysiology and application of monitoring to diabetesDiabetes Metab. Res. Rev.15(6)412-426(1999) 2.Espenshade, P.J., and Hughes, A.L.Regulation of sterol synthesis in eukaryotesAnnu. Rev. Genet.41401-427(2007) 3.Honda, A., Salen, G., Nguyen, L.B., et al.Regulation of early cholesterol biosynthesis in rat liver: Effects of sterols, bile acids, lovastatin, and BM 15.766 on 3-hydroxy-3-methylglutaryl coenzyme A synthase and acetoacetyl coenzyme A thiolase activitiesHepatology27(1)154-159(1998) 4.Berg, J.M., Tymoczko, J.L., and Stryer, L.Section 25.5 NAD+, FAD, and coenzyme A are formed from ATPBiochemistry5th Edition(2002)