3-demethyl Colchicine is an active metabolite of colchicine .1,2 It suppresses antibody production induced by sheep red blood cells in co-culture with isolated mouse spleen cells when used at concentrations ranging from 0.1 to 10 ?g/ml.2 3-demethyl Colchicine (100 ?g/animal) inhibits carrageenan-induced paw edema in rats.3
1.Sch?nharting, M., Mende, G., and Siebert, G.Metabolic transformation of colchicine. II. The metabolism of colchicine by mammalian liver microsomesHoppe Seylers Z. Physiol. Chem.355(11)1391-1399(1974) 2.Sterzl, J., Santav?, F., Sedmera, P., et al.Effect of colchicine derivatives on the antibody response induced in vitroFolia Microbiol. (Praha)27(4)256-266(1982) 3.Sugio, K., Maruyama, M., Tsurufuji, S., et al.Separation of tubulin-binding and anti-inflammatory activity in colchicine analogs and congenersLife Sci.40(1)35-39(1987)