DiO (3,3′-Dioctadecyloxacarbocyanine perchlorate) is a lipophilic long-chain carbocyanine fluorescent dye that is widely used as Di to label cells, organelles, liposomes, viruses and lipoproteins. 3,3′-Octadecyloxacarbocyanine perchlorate is a green fluorescent lipophilic tracer that is weakly fluorescent in water but highly fluorescent and comparable when incorporated into membranes Photostability. 3,3′-Octadecyloxacarbocyanine perchlorate is used as a fluorescent marker for living cells and as a lipophilic dye for labeling zebrafish embryos[1].
Long-chain carbocyanines include DiO, DiI, DiD, DiR, and the dialkylaminostyrene dye DiA for labeling membranes and other hydrophobic structures. DiO fluorochrome exhibits pronounced fluorescence, which facilitates multicolor imaging and flow cytometry analysis of living cells[2]. DiO can be used with standard FITC filters.
References:
[1]. Anat Samuel, et al. Six3 regulates optic nerve development via multiple mechanisms. 2016 Jan 29;6:20267. doi: 10.1038/srep20267.
[2]. Bhowmik BB, et al. Photophysical studies of 3,3' dioctadecyloxacarbocyanine dye in model biological membranes and different solvents. Chem Phys Lipids. 2001 Feb;109(2):175-83.
DiO（ 3,3′-Dioctadecyloxacarbocyanine perchlorate）是一种亲脂性长链羰花青荧光染料，被广泛用作Di来标记细胞，细胞器，脂质体，病毒和脂蛋白。3，3′-二十八烷基氧杂羰花青高氯酸盐是一种绿色荧光亲脂性示踪剂，在水中荧光较弱，但当掺入膜中时具有高荧光性和相当的光稳定性。3，3′-二十八烷基氧杂羰花青高氯酸盐被用作活细胞的荧光标记物，也被用作斑马鱼胚胎标记的亲脂性染料[1]。
长链羰花青包括DiO、DiI、DiD、DiR以及二烷基氨基苯乙烯染料DiA，用于标记膜和其他疏水结构。DiO荧光染料表现出明显的荧光，有助于活细胞的多色成像和流式细胞术分析[2]。DiO可与标准 FITC滤光片一起使用。