Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.
A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of iodoacetamide.[2]
Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). Iodoacetamide induces gastritis in rats.[3]
[1] Peter G Hains, Phillip J Robinson. J Proteome Res. 2017 Sep 1;16(9):3443-3447. [2] N F Moore, et al. Arch Virol. 1981;68(1):1-8. [3] Kata Csek?, et al. Int J Mol Sci. 2020 Aug 5;21(16):5591.