2′-O,4′-C-Methyleneguanosine (LNA-G) is a reverse guanine analogue, where LNA (locked nucleic acid) is a nucleic acid analogue. LNA modification can be used in a variety of applications such as effective binding affinity to complementary sequences and greater nuclease resistance than natural nucleotides, offering great potential for applications in disease diagnosis and research. LNA-G is also available via KOD DNA polymerase, which allows the integration of LNA-G nucleotides into the DNA strand[1][2].
[1]. Rakesh N. Veedu, et al. Polymerase directed incorporation studies of LNA-G nucleoside 5′-triphosphate and primer extension involving all four LNAnucleotides. New J. Chem., 2010,34, 877-879
[2]. Haase L, et al. Locked nucleic acid building blocks as versatile tools for advanced G-quadruplex design. Nucleic Acids Res. 2020 Oct 9;48(18):10555-10566.