15-deoxy-δ12,14-Prostaglandin J2-d4

15-deoxy-δ^12,14-PGJ₂-d₄ contains four deuterium atoms at the 3, 3', 4, and 4' positions. It is intended for use as an internal standard for the quantification of 15-deoxy-δ^12,14-PGJ₂ by GC- or LC-mass spectrometry. 15-deoxy-δ^12,14-Prostaglandin J₂ (15-deoxy-δ^12,14-PGJ₂) is a metabolite of PGJ₂. 15-deoxy-δ^12,14-PGJ₂ is formed from PGD₂ by the elimination of two molecules of water. It binds selectively to PPARγ with an EC₅₀ of 2 µM in a murine chimera system.^1,2 15-deoxy-δ^12,14-PGJ₂ is more potent than PGD₂, δ¹²-PGJ₂, and PGJ₂ in stimulating lipogenesis in C3H10T1/2 cells. The EC₅₀ for induction of adipocyte differentiation in cultured fibroblasts is 7 µM.¹

1.Forman, B.M., Tontonoz, P., Chen, J., et al.15-Deoxy-δ12,14-prostaglandin J2 is a ligand for the adipocyte determination factor PPARγCell83(5)803-812(1995) 2.Kliewer, S.A., Lenhard, J.M., Willson, T.M., et al.A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor γ and promotes adipocyte differentiationCell83(5)813-819(1995)