11-keto Testosterone is an oxidized form of testosterone and a non-aromatizable active androgen[1, 2]. 11-keto Testosterone is the major androgen in fish[3]. 11-keto Testosterone can promote the degeneration of oocytes, the proliferation of spermatocytes, and the formation of sperm in grouper[4].
In vitro, 11-keto Testosterone (10, 100μM) treatment of Sterlet liver explants and ovarian explants for 5 days significantly increased the concentrations of testosterone and 17β-estradiol in the culture medium of liver explants and ovarian explants, and the concentration of vitellogenin in the culture medium of liver explants also increased significantly[5].
In vivo, silicone rubber strips coated with 11-keto Testosterone (5, 25mg/kg/day) were implanted into pre-pregnant female Sterlet for 30 days and promoted ovarian development in a dose-dependent manner, but no ovarian masculinization was found[5].
References:
[1] Stárka L, Dušková M, Vítků J. 11-Keto-testosterone and other androgens of adrenal origin[J]. Physiological Research, 2020, 69(Suppl 2): S187.
[2] Felício A A, Crago J, Maryoung L A, et al. Effects of alkylphenols on the biotransformation of diuron and enzymes involved in the synthesis and clearance of sex steroids in juvenile male tilapia (Oreochromus mossambica)[J]. Aquatic Toxicology, 2016, 180: 345-352.
[3] Loggie J W. A test of the effects of androgens on immunity: no relationship between 11-keto testosterone and immune performance in bluegill sunfish (Lepomis macrochirus)[D]. The University of Western Ontario (Canada), 2016.
[4] Lee C H, Hur S W, Song Y B, et al. Effect of 11-ketotestosterone (11-KT) on gonadal sex reversal and spermatogenesis of honeycomb grouper Epinephelus merra[J]. Development and Reproduction, 2010, 14(1): 1-5.
[5] Wang W, Zhu H, Tian Z, et al. Effects of 11-Ketotestosterone on development of the previtellogenic ovary in the sterlet, Acipenser Ruthenus. Front Endocrinol (lausanne) 11: 115[J]. 2020.
11-keto Testosterone（11-酮基睾酮）是一种氧化形式的睾酮，是一种非芳香化的活性雄激素[1, 2]。11-keto Testosterone在鱼类中是主要的雄激素[3]。11-keto Testosterone能促进石斑鱼卵母细胞的变性、精母细胞的增殖和精子的形成[4]。
在体外，11-keto Testosterone（10, 100μM）处理Sterlet（小体鲟）的肝外植体和卵巢外植体5天，显著增加了肝外植体和卵巢外植体培养基中的睾酮和17β-雌二醇的浓度，肝外植体培养基中的卵黄蛋白原浓度也显著增加[5]。
在体内，11-keto Testosterone（5, 25mg/kg/day）包埋的硅橡胶条植入孕前期雌性Sterlet体内30天，以剂量依赖性方式促进了卵巢发育，未发现卵巢雄性化[5]。