11β-13,14-dihydro-15-keto Prostaglandin F2α-d9 (11β-13,14-dihydro-15-keto PGF2α-d9) is intended for use as an internal standard for the quantification of 11β-13,14-dihydro-15-keto PGF2α by GC- or LC-MS. 11β-13,14-dihydro-15-keto PGF2α is a metabolite of prostaglandin D2 in the 15-hydroxy PGDH pathway.1,2 Infusion or inhalation of tritiated PGD2 in normal males results in the appearance of peak levels of both 11β-PGF2α, as the immediate product, and 11β-13,14-dihydro-15-keto PGF2α in the plasma within 10 minutes.1 Homogenates of human lung metabolize PGD2 first to 11β-PGF2α and then to 11β-15-keto-PGF2α in the presence of NAD+, but not 11β-13,14-dihydro-15-keto PGF2α.2 Guinea pig liver and kidney homogenates metabolize PGD2 to 11β-13,14-dihydro-15-keto PGF2α, via 11β-PGF2α, in the presence of NAD+ and NADP+.2
1.Robinson, C., Hardy, C.C., and Holgate, S.T.The metabolism of prostaglandin D2 after inhalation or intravenous infusion in normal menBiochim. Biophys. Acta963(2)151-161(1988) 2.Robinson, C., Herbert, C.A., Bedwell, S., et al.The metabolism of prostaglandin D2. Evidence for the sequential conversion by NADPH and NAD+ dependent pathwaysBiochem. Pharmacol.38(19)3267-3271(1989)