1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine
(Synonyms: 1-十七碳酰-甘油-3-磷酰胆碱) 目录号 : GC26199
一键复制产品信息
1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine是血液中存在的一种内源性代谢物，可作为小鼠模型抑郁症的诊断生物标志物。
Cas No.:50930-23-9
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine is an endogenous metabolite present in the blood and can be used as a diagnostic biomarker for depression in mouse models [1]. 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine alters the rigidity of the cell membrane and can interfere with tumor metastasis[2]. 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine is widely used as an internal standard to develop electrospray mass spectrometry for the identification of related compounds [3].
In vivo, 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine treatment via a single intravenous injection at a dose of 5mg/kg for 2 hours significantly reduced the interaction between TRPV4 and KCa3.1 channels in the coronary artery endothelial cells (CAECs) of mice and decreased the vascular dilation of the arterial segments[4].
References:
[1] Wang Y, Chen X, Chen Z, et al. Disturbances of phosphatidylcholines metabolism in major depressive disorder[J]. CNS spectrums, 2023, 28(5): 637-645.
[2] 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine alters the rigidity of the cell membrane and can interfere with tumor metastasis.
[3] Rush M D, van Breemen R B. Role of ammonium in the ionization of phosphatidylcholines during electrospray mass spectrometry[J]. Rapid Communications in Mass Spectrometry, 2017, 31(3): 264-268.
[4] Mao A, He D, Zhang K, et al. Functional role of coupling the endothelial TRPV4 and KCa3. 1 channels in regulating coronary vascular tone[J]. British Journal of Pharmacology, 2023, 180(17): 2266-2279.
1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine是血液中存在的一种内源性代谢物，可作为小鼠模型抑郁症的诊断生物标志物[1]。1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine可改变细胞膜的刚性，并能干扰肿瘤转移[2]。1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine被广泛用作内标，以开发电喷雾质谱法用于鉴定相关化合物[3]。
在体内，单次静脉注射5mg/kg剂量的1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine，持续2小时，显著降低了小鼠冠状动脉内皮细胞(CAECs)中TRPV4与KCa3.1通道之间的相互作用，并减少了动脉段的血管舒张[4]。
| Animal experiment [1]: | |
Animal models | C57BL/6J mice |
Preparation Method | Male C57BL/6J mice (6 weeks old) were housed under specific-pathogen-free conditions, with environmentally controlled conditions (24±1°C, 12h light/dark cycles) with free access to dry food and water. Mice were injected i.v. with 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (5mg/kg) or vehicle. After 2h, mice were killed by CO2 asphyxiation and the coronary artery endothelial cells were collected for analysis. |
Dosage form | 5mg/kg for 4 once; i.v. |
Applications | 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine treatment significantly reduced the interaction between TRPV4 and KCa3.1 channels in the CAECs of mice. |
References: | |
| Cas No. | 50930-23-9 | SDF | |
| 别名 | 1-十七碳酰-甘油-3-磷酰胆碱 | ||
| 分子式 | C25H52NO7P | 分子量 | 509.66 |
| 溶解度 | Ethanol: 25 mg/ml | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.9621 mL | 9.8105 mL | 19.6209 mL |
| 5 mM | 392.4 μL | 1.9621 mL | 3.9242 mL |
| 10 mM | 196.2 μL | 981 μL | 1.9621 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet