1,3-Diphenylurea is a cytokinin.1,2 It increases fresh weight of tobacco plant tissue when used at concentrations ranging from 1 to 16 mg/L in growth media.1 1,3-Diphenylurea (32 and 100 µM) increases the growth of cultured P. lunatus callus tissue.2 Exogenous 1,3-diphenylurea (32 and 100 µM) added during first-passage culture induces second-passage cytokinin autonomy in cultured P. lunatus tissue.
1.Dyson, W.H., Fox, J.E., and McChesney, J.D.Short term metabolism of urea and purime cytokininsPlant Physiol.49(4)506-513(1970) 2.Mok, M.C., Kim, S.-G., Armstrong, D.J., et al.Induction of cytokinin autonomy by N,N'-diphenylurea in tissue cultures of Phaseolus lunatus L.Proc. Natl. Acad. Sci. USA76(8)3880-3884(1979)