ZnAF-2 DA是锌离子荧光探针ZnAF-2 的膜可渗透衍生物，一进入胞内，被细胞质内的酯酶水解生成ZnAF-2F，从而保留在胞内。ZnAF-2 DA适用于活细胞或组织(比如:海马薄片)的生物成像分析。ZnAF-2具有连接在TPEN类似物上的荧光素结构，在缓冲液中高度特异性结合Zn2+。高亲和力从而能用于检测低浓度Zn2+(解离常数:2.7nM) 。低荧光背景从而高灵敏度测定活样本内Zn2+。ZnAF-2与Zn2+结合后发绿色荧光 (Ex/Em=492/515nm) [1-3]。
References:
[1] Highly Zinc-Selective Fluorescent Sensor Molecules Suitable for Biological Applications: T. Hirano, et al; JACS 122,12399 (2000)
[2] Mossy fiber Zn2+ spilover modulates heterosynaptic N-methy-D-aspartate receptor activity in hippocampal CA3 circuits: S.Ueno, et al; J. Cell Biol, 158, 215 (2002)
[3] lmprovement and biological applications of fluorescent probes for zinc, ZnAFs: T. Hirano, et al,; JACS 124, 6555 (2002)
Fig 1. ntracelular elevation of dual Zn-Mn ions and ROS by Mn-Zn02 NPs. (a) Schematic ilustration of intracellular release of dualZn-Mn ions and H202 and subsequent 0H generation from Mn-Zn02 NPs. (b) Fluorescence images of MDA-MB-231 cells stainedwith Zn2+ dye- ZnAF-2 DA (green, 5 uM ) after incubation with ZnCl2 or Mn-Zn02 NPs for 4 h.
a)Fig Fig 2 (a) Bright-field and (b, c) fluorescence images of RAW 2647 cells incubated with 10 mlM ZnAF-2F DA. (d) Changes in the average fluorescence intensity of the coresponding areas (1-3, intrace ular redion: 4. extrace ular redion) as a function of time, The fluorescence excited at 470-490 nm was measured at the intervals of 20 s. At 2 min, Zn 2+-pyrithione were added to the medium, and at 10 min 100 mM TPEN was added.