Zinquin ethyl ester is a lipophilic, cell membrane-permeable fluorescent probe for zinc ions (excitation wavelength 368nm, emission wavelength 490nm). After entering cells, Zinquin ethyl ester is hydrolyzed by esterases to generate negatively charged Zinquin, which remains intracellularly and forms a complex with Zn²⁺, resulting in enhanced fluorescence. Zinquin ethyl ester is excited by ultraviolet light and emits fluorescence in the blue spectral range to detect changes in intracellular free zinc ion concentration. Zinquin ethyl ester can be used in research related to cell biology, neuroscience, and apoptosis-associated zinc ion flux changes[1-4].
References:
[1] Nowakowski AB, Petering DH. Reactions of the fluorescent sensor, Zinquin, with the zinc-proteome: adduct formation and ligand substitution. Inorg Chem. 2011 Oct 17;50(20):10124-33.
[2] Kimber MC, Mahadevan IB, Lincoln SF, et al. The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester. J Org Chem. 2000 Dec 1;65(24):8204-9.
[3] Merten KE, Jiang Y, Kang YJ. Zinc inhibits doxorubicin-activated calcineurin signal transduction pathway in H9c2 embryonic rat cardiac cells. Exp Biol Med (Maywood). 2007 May;232(5):682-9.
[4] Song W, Tang X, Li Y, et al. In situ detection of the Zn(2+) release process of ZnO NPs in tumour cells by confocal laser scanning fluorescence microscopy. IET Nanobiotechnol. 2016 Aug;10(4):178-83.
Zinquin ethyl ester是一种亲脂性的、具有细胞膜穿透性的锌离子荧光探针（激发波长为368nm，发射波长490nm）。Zinquin ethyl ester可进入细胞后被酯酶水解生成带负电荷的Zinquin来滞留在胞内并与Zn²⁺形成复合物产生荧光增强，同时通过紫外激发在蓝色光谱范围发射荧光以检测细胞内游离锌离子浓度变化。Zinquin ethyl ester可用于细胞生物学、神经科学和凋亡相关锌离子流量变化的相关研究[1-4]。