Zinc (Zn)-fluorophore 1(Zinpyr-1, ZP1), a fluorescent probe for zinc detection, emits bright fluorescence has been used extensively for zinc labeling (Ex/Em: 515nm/531nm) (ε =79,000/M/cm; pKa = 8.4; Φ = 0.38). Zinpyr-1 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry, and can be used for live and fixed cell applications. Zinpyr-1 is widely applied in vitro, ex vivo, and in vivo materials and models from animals, plants, and bacteria, such as human serum, arabidopsis roots, rats, and Escherichia coli.[1]. Zinpyr-1 is composed of a fluorescein-derivative reporter and metal-binding units, enabling Zinpyr-1 to bind Zn2+ while emissing fluoresce signal [2].
References:
[1] Sinclair, Scott A et al. “The use of the zinc-fluorophore, Zinpyr-1, in the study of zinc homeostasis in Arabidopsis roots.” *The New phytologist* vol. 174,1 (2007): 39-45. doi:10.1111/j.1469-8137.2007.02030.x
[2] Goldsmith, Christian R, and Stephen J Lippard. “Analogues of Zinpyr-1 provide insight into the mechanism of zinc sensing.” *Inorganic chemistry* vol. 45,16 (2006): 6474-8. doi:10.1021/ic060378j
Zinc (Zn)-fluorophore 1（Zinpyr-1, ZP1）是一种用于锌检测的荧光探针，它发出明亮的荧光，被广泛用于锌标记（Ex/Em: 515nm/531nm） （ε =79,000/M/cm; pKa = 8.4; Φ = 0.38）。Zinpyr-1兼容聚光，共聚焦和双光子显微镜，以及流式细胞术，并可用于活细胞和固定细胞的应用。Zinpyr-1广泛应用于动物、植物和细菌的离体、离体和体内材料和模型，如人血清、拟南芥根、大鼠、大肠杆菌等[1]。Zinpyr-1由荧光素衍生物报告因子和金属结合单元组成，使Zinpyr-1在结合Zn2+的同时发出荧光信号[2]。