WSP-5 (Washington State Probe-5) is a fluorescent probe containing active disulfide groups, specifically designed for rapid detection of hydrogen sulfide (H2S) in biological samples and within cells. H2S, like nitric oxide, has significant effects on mammalian immune, nervous, cardiovascular, and pulmonary systems. Through a reaction-based fluorescence turn-on strategy, WSP-5 selectively and rapidly reacts with H2S, generating fluorescence with excitation and emission maxima at 502 and 525 nm, respectively[1]. Compared with the three H2S probes WSP-1, CAY and P3, WSP-5 has the widest linear range of sensitivity, minimal interference to thiols and other analytes, the most stable in different pH environments, and can be used in Imaging H2S in live cell systems [2].
References:
[1] Peng, B., Chen, W., Liu, C., et al. Fluorescent probes based on nucleophilic substitution-cyclization for hydrogen sulfide detection and bioimaging[J]. Chemistry 20(4), 1010-1016 (2014).
[2] Yingzhu Zhou, Dr. Federico Mazur,et al. Sensitivity and Selectivity Analysis of Fluorescent Probes for Hydrogen Sulfide Detection[J]. Chemistry – An Asian Journal
March 1, 2022.
WSP-5(Washington State Probe-5)是一种含活性二硫化物的荧光探针，特别设计用来快速检测生物样本和细胞内的硫化氢(H2S)。H2S是一种重要的气体介质，与一氧化氮一样，对哺乳动物的免疫、神经、心血管和肺系统具有显著影响。通过基于反应的荧光开启策略，WSP-5选择性地和快速地与H2S反应，产生激发和发射最大值分别为502和525 nm的荧光团[1]。与WSP-1、CAY和P3这三种H2S探针相比，WSP-5具有最宽的灵敏度线性范围、对硫醇和其他分析物干扰最小、在不同pH环境中最稳定等优点，并且能够在活细胞系统中对H2S成像[2]。