Violuric acid is a redox mediator used in the laccase system. The violuric acid assay is a method to ascertain that the high-redox potential of laccase is not lost during directed evolution[1][2].
References:
[1]. Zhenyu Shi, et al. Enhanced Transformation of Emerging Contaminants by Permanganate in the Presence of Redox Mediators. Environ Sci Technol. 2020 Feb 4;54(3):1909-1919.
[2]. Isabel Pardo, et al. Colorimetric High-Throughput Screening Assays for the Directed Evolution of Fungal Laccases. Methods Mol Biol. 2018;1685:247-254.
















