Vincristine-d3 is intended for use as an internal standard for the quantification of vincristine by GC- or LC-MS. Vincristine is a vinca alkaloid that has been found in C. rosea and has anticancer activities.[1],[2] It inhibits tubulin polymerization in a cell-free assay (Ki = 0.085 µM) and induces mitotic arrest in U937 cells when used at a concentration of 10 nM. In vivo, vincristine (3 mg/kg) reduces tumor growth in rhabdomyosarcoma patient-derived xenograft (PDX) mouse models.[3] Formulations containing vincristine have been used in the treatment of various cancers.
References:
[1].Jordan, M.A., Himes, R.H., and Wilson, L.Comparison of the effects of vinblastine, vincristine, vindesine, and vinepidine on microtubule dynamics and cell proliferation in vitroCancer Res.45(6)2741-2747(1985).
[2].Towle, M.J., Salvato, K.A., Wels, B.F., et al.Eribulin induces irreversible mitotic blockade: Implications of cell-based pharmacodynamics for in vivo efficacy under intermittent dosing conditionsCancer Res.71(2)496-505(2011).
[3].Horton, J.K., Houghton, P.J., and Houghton, J.A.Relationships between tumor responsiveness, vincristine pharmacokinetics and arrest of mitosis in human tumor xenograftsBiochem. Pharmacol.37(20)3995-4000(1988).