Tyrosinase (From mushrooms, ≥1000 units/mg dry weight) is an enzyme that catalyzes the oxidation of phenolic compounds (such as tyrosine, phenolic compounds, etc.) into quinones[1]. Tyrosinase is a copper-containing enzyme found in animal and plant tissues that catalyzes the oxidation of tyrosine to produce melanin and other pigments. It is present in melanosomes synthesized by skin melanocytes and is encoded by the TYR gene[2]. Tyrosinase is widely present in nature and is usually extracted from wild mushrooms[3]. Tyrosinase is also involved in the browning reaction, causing damaged fruits or plant tissues to turn brown[4]. Tyrosinase contains copper ions, with a copper content of about 0.25%[5]. The ultraviolet absorption peaks of Tyrosinase are at 273nm and 330nm[6]. The molecular weight of this product is about 128kDa, the specific activity is ≥1000units/mg dry weight, and the optimal pH is 6.0-7.0.
References:
[1] Duckworth H W. Towards a Mechanism of Action for Tyrosinase[M]. Yale University, 1970.
[2] Abdelrasool M, Said A. Literature review on tyrosinase enzyme[J]. 2017.
[3] Seo S Y, Sharma V K, Sharma N. Mushroom tyrosinase: recent prospects[J]. Journal of agricultural and food chemistry, 2003, 51(10): 2837-2853.
[4] Singh B, Suri K, Shevkani K, et al. Enzymatic browning of fruit and vegetables: A review[J]. Enzymes in food technology: Improvements and innovations, 2018: 63-78.
[5] Makino N, McMahill P, Mason H S, et al. The oxidation state of copper in resting tyrosinase[J]. Journal of Biological Chemistry, 1974, 249(19): 6062-6066.
[6] Rojas J, Londoño C, Ciro Y. The health benefits of natural skin UVA photoprotective compounds found in botanical sources[J]. Int J Pharm Pharm Sci, 2016, 8(3): 13-23.
Tyrosinase (From mushrooms, ≥1000 units/mg dry weight) 是一种可催化含酚基化合物(如酪氨酸、酚类化合物等)氧化成醌的酶[1]。Tyrosinase是一种存在于动植物组织中的含铜酶，可催化酪氨酸氧化产生黑色素和其他色素，它存在于皮肤黑素细胞合成的黑素体内，由TYR基因编码[2]。Tyrosinase广泛存在于自然界，通常从野蘑菇中提取[3]。Tyrosinase还参与褐变反应，导致受损水果或植物组织褐变[4]。Tyrosinase含有铜离子，含铜量约0.25％[5]。Tyrosinase的紫外吸收峰在273nm和330nm处[6]。本产品的分子量约128kDa，比活力≥1000units/mg dry weight，最适pH为6.0-7.0。