TSQ is a fluorescent probe that images zinc proteins in cells, with maximum excitation/emission wavelengths near 360nm/470nm[1]. TSQ was successfully employed in pinpoint a pool of free Zn2+ in the brain[2]. TSQ functions as an effective tool for evaluation of Zn2+ within artificial membranes and sperm cells while demonstrating strong binding to lipid bilayers[3]. TSQ can be used to detect free zinc in breast cells (MCF7, MDA-MB-231, T47D, and MCF10A cells) and to determine the distribution and uptake of zinc[4]. TSQ can be used for histochemical staining of tissue sections, which can show the location of zinc-containing (glutamatergic) nerve endings in neurofibroma[5].
References:
[1] Meeusen J W, Tomasiewicz H, Nowakowski A, et al. TSQ (6-methoxy-8-p-toluenesulfonamido-quinoline), a common fluorescent sensor for cellular zinc, images zinc proteins[J]. Inorganic chemistry, 2011, 50(16): 7563-7573.
[2] Frederickson C J, Kasarskis E J, Ringo D, et al. A quinoline fluorescence method for visualizing and assaying the histochemically reactive zinc (bouton zinc) in the brain[J]. Journal of neuroscience methods, 1987, 20(2): 91-103.
[3] Andrews J C, Nolan J P, Hammerstedt R H, et al. Characterization of N‐(6‐methoxy‐8‐quinolyl)‐p‐toluenesulfonamide for the detection of zinc in living sperm cells[J]. Cytometry: The Journal of the International Society for Analytical Cytology, 1995, 21(2): 153-159.
[4] Hübner C, Keil C, Jürgensen A, et al. Comparison of three low-molecular-weight fluorescent probes for measuring free zinc levels in cultured mammary cells[J]. Nutrients, 2023, 15(8): 1873.
[5] Frederickson C J, Suh S W, Silva D, et al. Importance of zinc in the central nervous system: the zinc-containing neuron[J]. The Journal of nutrition, 2000, 130(5): 1471S-1483S.
TSQ是一种能显示细胞内锌蛋白的荧光探针，最大激发/发射波长在360nm/470nm附近[1]。TSQ已成功应用于脑部游离Zn2+的精确定位[2]。TSQ能有效用于评估人工膜和精子细胞内的Zn2+，表现出与脂质双分子层的强结合特性[3]。TSQ可用于检测乳腺癌细胞（MCF7、MDA-MB-231、T47D和MCF10A细胞）中的游离锌，并测定锌的分布与摄取情况[4]。TSQ可用于组织切片的组织化学染色，能显示神经纤维瘤中含锌（谷氨酸能）神经末梢的定位[5]。