TMB (BM blue) is a novel, safe, non-toxic, and non-mutagenic colorless dye, TMB (BM blue) serves as a chromogenic agent for horseradish peroxidase (HRP)-based detection systems and is commonly used as a chromogenic substrate in immunohistochemical staining procedures and enzyme-linked immunosorbent assay (ELISA)[1]. TMB can act as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products[2].
References:
[1] Fanjul-Bolado P, González-García MB, Costa-García A. Amperometric detection in TMB/HRP-based assays. Anal Bioanal Chem. 2005 May;382(2):297-302.
[2] Jhaveri S, Carman L, Hahm JO. Visualizing anterogradely transported HRP by use of TMB histochemistry: comparison of the TMB-SNF and TMB-AHM methods. J Histochem Cytochem. 1988 Jan;36(1):103-5.
TMB (BM blue) 是一种新型安全的无毒且不致突变的无色染料，也是用于基于辣根过氧化物酶（HRP）检测系统的显色剂，常作为免疫组织化学染色程序和酶联免疫吸附测定（ELISA）的显色底物[1]。TMB可以作为活性氧和氮物质（ROS/RNS）光声探针，与次氯酸盐、过氧化氢、单线态氧和二氧化氮等反应产生蓝色氧化产物[2]。