Amine reactive orange fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, 2-photon excitation microscopy, and super resolution mircoscopy techniques, such as dSTORM. Excitation maximum = 578 nm; emission maximum = 602 nm; quantum yield = 0.69; extinction coefficient = 60720 M-1cm-1.
Panchuk-Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID:10449539 |Van de Linde et al (2011) Direct stochastic optical reconstruction microscopy with standard fluorescent probes Nature Protocols 6 991 |Rossi et al (2012) Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes. J.Cell Sci. 125 4405 PMID:22718347