Amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Less susceptible to spontaneous hydrolysis during conjugation reactions compared to SE or NHS dyes. Also displays greater stability compared to NHS and SE versions, typically lasting several hours. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.
Schilling et al (2006) On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry 45 12393 PMID:17029395