Tempone-H may be used as a spin trap in chemical and biological systems to quantify peroxynitrite and superoxide radical formation. Ferric and cupric ions are effective oxidants of Tempone-H.
Using TEMPONE-H the obtained sensitivity in the detection of peroxynitrite or superoxide radicals is about 10-fold higher than using the spin traps DMPO or TMIO[1].
References:
[1]. Dikalov S, et al. Quantification of peroxynitrite, superoxide, and peroxyl radicals by a new spin trap hydroxylamine 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine. Biochem Biophys Res Commun. 1997 Jan 3;230(1):54-7.
[2]. Dikalov SI, et al. Amyloid beta peptides do not form peptide-derived free radicals spontaneously, but can enhance metal-catalyzed oxidation of hydroxylamines to nitroxides. J Biol Chem. 1999 Apr 2;274(14):9392-9.