Tectochrysin (Techtochrysin)

Cell experiment:

Each SW480, HCT116, HT-29, A549 and MCF-7 cell line (1×104 cells) is incubated in 200 μL of RPMI 1640, DMEM medium with NSC 80687 (concentrations ranging from 1, 5, 10 μg/mL) in a 96-well flat-bottomed plate in triplicate. After incubation for 72 h at 37°C, MTT diluted in RPMI 1640, DMEM medium are added to each well and incubation is carried out for 90 min. The supernatant is then discarded and the crystal products are eluted with DMSO (200 μL/well). Colorimetric evaluation is performed with a spectrophotometer at 540 nm. The apoptosis assay is first performed by using DAPI staining. SW480 and HCT116 human colon cancer cells are cultured with concentrations of NSC 80687 (5 μg/mL), and induction of apoptotic cell death is evaluated after 24 h. Tunel assay is done[1].

Animal experiment:

Mice[1]Five-week-old male BALB/c athymic nude mice (n=10/group) are used. HCT116 cancer cells are injected subcutaneously (1×107 cells/0.1 mL PBS/animal) into the lower right flanks of mice. After 14 days, when the tumors have reached an average volume of 200-300 mm3, the tumor-bearing nude mice are intraperitoneally injected with Tectochrysin (NSC 80687) (5 mg/kg dissolved in 0.1 % DMSO) twice per week for 3 weeks. The tumor volumes are measured with vernier calipers and calculated[1].

References:

[1]. Park MH, et al. Anticancer effect of Tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression. Mol Cancer. 2015 Jun 30;14:124.