TC-T 6000

目录号: GC50098纯度: >99.50%

TC-T 6000是一种有效的、选择性的人平衡核苷转运蛋白4（ENT4）抑制剂，IC₅₀为74.4nM。

Cas No.: 949467-71-4

规格	价格	库存	数量
1mg	¥509.00	现货	1
5mg	¥1,120.00	现货	1
10mg	¥1,960.00	现货	1
25mg	¥3,920.00	现货	1
50mg	¥6,160.00	现货	1
10mM (in 1mL DMSO)	¥1,232.00	现货	1

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文献被引

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632, 686–694 (2024)
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618, 1017–1023 (2023)
Nature
610, 366–372 (2022)
Cell
187(9):2288-2304 (2024)
Cell
183(7):1867-1883 (2020)
Science
388(6745) (2025)
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387(6739) (2025)
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387(6734) (2025)
Cell Research
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33, 546–561 (2023)
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33, 904–922 (2023)
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产品描述 Description

TC-T 6000, a Dipyridamole analog, is a potent and selective human ENT4 (Balanced nucleoside transporter 4) inhibitor with an IC₅₀ of 74.4nM. Making it about 38 times more potent than Dipyridamole^[1].

TC-T 6000(10μM; 24h) shows no cytotoxicity against PK15/hENT4 cells.

References:
[1]. Wang C, Lin W, et,al. Dipyridamole analogs as pharmacological inhibitors of equilibrative nucleoside transporters. Identification of novel potent and selective inhibitors of the adenosine transporter function of human equilibrative nucleoside transporter 4 (hENT4). Biochem Pharmacol. 2013 Dec 1;86(11):1531-40. doi: 10.1016/j.bcp.2013.08.063. Epub 2013 Sep 7. PMID: 24021350; PMCID: PMC3866046.

TC-T 6000是一种有效的、选择性的人平衡核苷转运蛋白4（ENT4）抑制剂，IC₅₀为74.4nM。TC-T 6000比双嘧达莫有效约38倍^[1]。

TC-T 6000(10μM;24h)对PK15/hENT4细胞无细胞毒性。

实验参考方法 Experimental Reference Method

Cell experiment [1]:
Cell lines	PK15/hENT cells (PK15 cells stably expressing hENT4)
Preparation Method	Cells were seeded at 5×10³ cells were seeded in 96-well plates, treated with 10μM test TC-T 6000 and incubated at 37 ℃ in phenol-free MEM for 24h.
Reaction Conditions	10μM; 24h
Applications	TC-T 6000 shows no cytotoxicity against PK15/hENT4 cells.
Transporter inhibition assays [1]:
Preparation Method	Transport activity of cells transiently transfected with hENT4 was examined with [³H] Adenosine. K15/hENT4 cells were incubated with transport buffer containing TC-T 6000 at pH 6.0 for 15min before addition of 0.2μM [³H] Adenosine, and then further incubation for 2min. Uptake was terminated by rapidly aspirating the incubation mixture and washing cells three times with ice-cold PBS. The cells were solubilized overnight in 400μl of 5% Triton X-100 and 200μl of cell lysate was counted using a scintillation counter. Protein concentration in the cell lysate was determined using the BCA protein assay kit. The concentrations of test compounds that caused 50% inhibition of [³H] Adenosine uptake (IC₅₀) were calculated using a nonlinear fitting method in the Prism 4 program.
Reaction Conditions	10μM; 15min
Applications	TC-T 6000 is a potent and selective human ENT4 (Balanced nucleoside transporter 4) inhibitor with an IC₅₀ of 74.4nM.
References: [1]. Wang C, Lin W, et,al. Dipyridamole analogs as pharmacological inhibitors of equilibrative nucleoside transporters. Identification of novel potent and selective inhibitors of the Adenosine transporter function of human equilibrative nucleoside transporter 4 (hENT4). Biochem Pharmacol. 2013 Dec 1;86(11):1531-40. doi: 10.1016/j.bcp.2013.08.063. Epub 2013 Sep 7. PMID: 24021350; PMCID: PMC3866046.

产品文档 Product Documents

批次：Purity:>99.50%

化学性质Chemical Properties

CAS 号

949467-71-4

SMILES

CC(C)CN(CC(C)C)C2=NC(NCCO)=NC1=C(N(CC(C)C)CC(C)C)N=C(NCCO)N=C12

分子式

C₂₆H₄₈N₈O₂

分子量

504.71 g/mol

溶解性

Soluble in DMSO

保存条件

Store at -20°C

General tips

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。

Shipping Condition

评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

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