Tauro-β-muricholic acid-d4 (TβMCA-d4) is intended for use as an internal standard for the quantification of TβMCA by GC- or LC-MS. TβMCA is a competitive and reversible antagonist of the farnesoid X receptor (FXR; IC50 = 40 µM) and a taurine-conjugated form of the murine-specific primary bile acid β-muricholic acid .1 TβMCA accumulates in germ-free mice under normal conditions but is reduced after colonization with feces from a human donor.1,2 TβMCA is increased in the intestines of mice resistant to high-fat diet-induced obesity, fatty liver, and diabetes.3
1.Sayin, S.I., WahlstrÖm, A., Felin, J., et al.Gut microbiota regulates bile acid metabolism by reducing the levels of tauro-beta-muricholic acid, a naturally occurring FXR antagonistCell Metab.17(2)225-235(2013) 2.WahlstrÖm, A., Kovatcheva-Datchary, P., StÅhlman, M., et al.Induction of farnesoid X receptor signaling in germ-free mice colonized with a human microbiotaJ. Lipid. Res.58(2)412-419(2017) 3.Qi, Y., Jiang, C., Cheng, J., et al.Bile acid signaling in lipid metabolism: Metabolomic and lipidomic analysis of lipid and bile acid markers linked to anti-obesity and anti-diabetes in miceBiochim. Biophys. Acta.1851(1)19-29(2015)