Sulfo-NHS-Biotin is an amine-reactive biotinylation reagent, mainly used for simple and effective biotinylation labeling of antibodies, proteins and other macromolecules containing primary amines (-NH2)[1]. Sulfo-NHS-Biotin is a simple NHS ester-activated biotinylation reagent composed of natural biotin valerate groups with an arm length of 13.5A[2]. Sulfo-NHS-Biotin is a water-soluble analog obtained by adding a negatively charged sulfonic acid group to the NHS-biotin structure[3]. Sulfo-NHS-Biotin can label surface proteins of the entire cell without penetrating the cell membrane[4].
References:
[1] Elia G. Biotinylation reagents for the study of cell surface proteins[J]. Proteomics, 2008, 8(19): 4012-4024.
[2]Cohen L, Walt D R. Evaluation of antibody biotinylation approaches for enhanced sensitivity of single molecule array (Simoa) immunoassays[J]. Bioconjugate Chemistry, 2018, 29(10): 3452-3458.
[3] Ahmed K K, Geary S M, Salem A K. Engineering the Surface of Cells Using Biotin–Avidin Chemistry[M]Micro-and Nanoengineering of the Cell Surface. William Andrew Publishing, 2014: 143-155.
[4]Reglero-Real N, Pérez-Gutiérrez L, Yoshimura A, et al. Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation[J]. Immunity, 2021, 54(9): 1989-2004. e9.
Sulfo-NHS-Biotin是一种胺反应性生物素化试剂，主要用于对抗体、蛋白质和其他含伯胺(-NH2) 的大分子进行简单有效的生物素化标记[1]。Sulfo-NHS-Biotin是一种简单NHS酯活化的生物素化试剂，由天然生物素戊酸基团组成，臂长13.5A[2]。Sulfo-NHS-Biotin 是一种水溶性类似物，是通过在NHS-biotin结构上添加带负电荷的磺酸基而获得的[3]。Sulfo-NHS-Biotin可以对整个细胞的表面蛋白进行标记，不会渗透细胞膜[4]。