Sudan Red 7B

目录号: GC48625纯度: >98.00%同义词: 苏丹红7B
Sudan Red 7B是一种非荧光、脂溶性的偶氮染料,最大吸收波长为525nm。

Sudan Red 7B
Cas No.: 6368-72-5
规格价格库存数量操作
5g¥448.00现货
1
10g¥616.00现货
1
25g¥1,302.00现货
1
50g¥1,946.00现货
1

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产品描述 Description

Sudan Red 7B is a non-fluorescent, fat-soluble azo dye with a maximum absorption wavelength of 525nm[1, 2]. Sudan Red 7B can be used to stain fat in plant and animal tissues and can also indicate the presence of suberin in cell walls[3, 4]. Sudan Red 7B is also used as a standard for the detection of banned colorants in food[5].

References:
[1] Firoozeh F, Shahamat Y D, Rodríguez-Couto S, et al. Bioremediation for the Decolorization of Textile Dyes by Bacterial Strains Isolated from Dyeing Wastewater[J]. Jordan Journal of Biological Sciences, 2022, 15(2).
[2] Hassan Q M A, Manshad R K H. Optical limiting properties of sudan red B in solution and solid film[J]. Optical and Quantum Electronics, 2015, 47: 297-311.
[3] Mozgová I, Muñoz-Viana R, Hennig L. PRC2 represses hormone-induced somatic embryogenesis in vegetative tissue of Arabidopsis thaliana[J]. PLoS Genetics, 2017, 13(1): e1006562.
[4] Yang C, Zhang X, Wang T, et al. Phenotypic plasticity in the structure of fine adventitious Metasequoia glyptostroboides roots allows adaptation to aquatic and terrestrial environments[J]. Plants, 2019, 8(11): 501.
[5] Pardo O, Yusà V, León N, et al. Development of a method for the analysis of seven banned azo-dyes in chilli and hot chilli food samples by pressurised liquid extraction and liquid chromatography with electrospray ionization-tandem mass spectrometry[J]. Talanta, 2009, 78(1): 178-186.

Sudan Red 7B是一种非荧光、脂溶性的偶氮染料,最大吸收波长为525nm[1, 2]。Sudan Red 7B可用于对植物和动物组织中的脂肪进行染色,还可以指示细胞壁中是否存在木栓质[3, 4]。Sudan Red 7B还被用作检测食品中的禁用着色剂的标准品[5]

实验参考方法 Experimental Reference Method

本方案仅提供一个指导,请根据您的具体需要进行修改。

1. 溶液配制

(1)工作液:用DMF或DMSO溶解Sudan Red 7B,用实验缓冲液稀释储存液,配制到所需的工作浓度。

注意:最佳的工作浓度请根据实际情况调整或参阅文献自行设置梯度浓度进行摸索。工作液必须现配现用。

2. 使用Sudan Red 7B检测胚乳角质层结构[1](来源文献,仅供参考)

(1)样品在4°C的磷酸盐缓冲液(pH 7.2,含4%甲醛和0.25%戊二醛)中过夜固定。在固定开始时用细针刺穿干种子,所有样品均在真空下浸润。然后将样品嵌入1.5%琼脂糖颗粒中,在分级乙醇系列中脱水,包埋在石蜡中。用切片机切割12μm厚的切片。

(2)使用溶解在聚乙二醇400和90%(v/v)甘油1:1(v/v)的混合物中,终浓度为0.1%(w/v)的Sudan Red 7B孵育切片15min。

(3)用水清洗切片并封固在甘油中。用显微镜检查样品。

注意:为了您的安全和健康,请穿实验服并戴一次性手套操作。

References:

[1]Loubéry S, De Giorgi J, Utz-Pugin A, et al. A maternally deposited endosperm cuticle contributes to the physiological defects of transparent testa seeds[J]. Plant physiology, 2018, 177(3): 1218-1233.

产品文档 Product Documents

Purity:>98.00%

化学性质Chemical Properties

CAS 号
6368-72-5
同义词
苏丹红7B
SMILES
CCNC(C=CC1=C2C=CC=C1)=C2N=NC(C=C3)=CC=C3N=NC4=CC=CC=C4
分子式
C24H21N5
分子量
379.5 g/mol
溶解性
DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml,Ethanol: 1 mg/ml
保存条件
-20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至 37°C,然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备 RT,或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol