Sphingomyelins (buttermilk)是一种从酪乳中分离的、具有可变脂肪酸链长度的鞘磷脂混合物。
Cas No.:475662-40-9
Sample solution is provided at 25 µL, 10mM.
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Sphingomyelins (buttermilk) is a sphingomyelin mixture with variable fatty acid chain length, isolated from buttermilk. Sphingomyelins interacts with cholesterol to help maintain cellular cholesterol homeostasis, while also being hydrolyzed by sphingomyelinase to form ceramide, which mediates intracellular signaling[1-2]. Sphingomyelins can be used in research related to sphingolipid compounds and their roles in various biological processes[3-4].
In vitro, Vero-E6 epithelial cells were pretreated with Sphingomyelins (50μM) for 1 hour, followed by infection with pp-VSV-SARS-CoV-2 spike pseudoviral particles. Sphingomyelins had no significant effect on viral infection[5].
In vivo, PPAR-γ+/+ and PPAR-γ-/- mice were treated with Sphingomyelin (1g/kg; oral administration) for 80 days. Sphingomyelin significantly suppressed DSS-induced colon inflammation, increased mouse survival, and reduced AOM-induced tumor area[6]. 4-week-old male Jcl:ICR mice were treated with Sphingomyelins (0.1% in diet) for 10 days, while colitis was induced with 3% dextran sodium sulfate (DSS). Sphingomyelins significantly reduced the disease activity index (DAI) score, inhibited the increase in myeloperoxidase (MPO) activity in colon tissue, and increased the IgA content in the contents of the large intestine[7].
References:
[1] Kobayashi T. Mapping trasmembrane distribution of sphingomyelin. Emerg Top Life Sci. 2023 Mar 31;7(1):31-45.
[2] Signorelli P, Conte C, Albi E. The Multiple Roles of Sphingomyelin in Parkinson's Disease. Biomolecules. 2021 Sep 5;11(9):1311.
[3] Slotte JP. Biological functions of sphingomyelins. Prog Lipid Res. 2013 Oct;52(4):424-37.
[4] Exon JH, South EH. Effects of sphingomyelin on aberrant colonic crypt foci development, colon crypt cell proliferation and immune function in an aging rat tumor model. Food Chem Toxicol. 2003 Apr;41(4):471-6.
[5] Carpinteiro A, Gripp B, Hoffmann M, et al. Inhibition of acid sphingomyelinase by ambroxol prevents SARS-CoV-2 entry into epithelial cells. J Biol Chem. 2021 Jan-Jun;296:100701.
[6] Mazzei JC, Zhou H, Brayfield BP, et al. Suppression of intestinal inflammation and inflammation-driven colon cancer in mice by dietary sphingomyelin: importance of peroxisome proliferator-activated receptor γ expression. J Nutr Biochem. 2011 Dec;22(12):1160-71.
[7] Furuya H, Ohkawara S, Nagashima K, et al. Dietary sphingomyelin alleviates experimental inflammatory bowel disease in mice. Int J Vitam Nutr Res. 2008 Jan;78(1):41-9.
Sphingomyelins (buttermilk)是一种从酪乳中分离的、具有可变脂肪酸链长度的鞘磷脂混合物。Sphingomyelins通过与胆固醇相互作用，维持细胞胆固醇稳态，同时通过鞘磷脂酶水解形成神经酰胺以介导细胞内信号传导[1-2]。Sphingomyelins可用于鞘脂类化合物及其在多种生物学过程中作用的相关研究[3-4]。
在体外，Sphingomyelins（50μM）预处理Vero-E6上皮细胞1小时，随后以pp-VSV-SARS-CoV-2 spike假病毒颗粒感染细胞。Sphingomyelins对病毒的感染无显著影响[5]。
在体内，Sphingomyelin（1g/kg；口服）用于处理PPAR-γ+/+和PPAR-γ-/-小鼠，持续80天。Sphingomyelin显著抑制了DSS诱导的结肠炎症，增加了小鼠存活率，并减少了AOM诱导的肿瘤面积[6]。Sphingomyelins（0.1% in diet）用于处理4周龄雄性Jcl:ICR小鼠10天，同时以3%葡聚糖硫酸钠（DSS）诱导结肠炎。Sphingomyelins显著降低了疾病活动指数（DAI）评分，抑制了结肠组织髓过氧化物酶（MPO）活性的增加，并使大肠内容物中IgA的含量增加[7]。
| Cell experiment [1]: | |
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Cell lines |
Vero-E6 cells (African green monkey kidney epithelial cell line) |
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Preparation Method |
Vero-E6 cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS). Cells were treated with Sphingomyelins (50μM). |
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Reaction Conditions |
50μM; 1h pretreatment maintained during infection. |
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Applications |
Sphingomyelins pretreatment had no significant effect on the infection of cells with pp-VSV-SARS-CoV-2 spike pseudoviral particles. |
| Animal experiment [2]: | |
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Animal models |
4-week-old male Jcl:ICR mice |
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Preparation Method |
Mice were prefed a sphingolipid-free AIN-76 diet for 3 days, then fed the AIN-76 diet supplemented with 0.1% (wt/wt) Sphingomyelins for 10 days. From day 3 of Sphingomyelins feeding, 3% (wt/v) dextran sodium sulfate (DSS) was administered in drinking water for 7 days. Mice were sacrificed on day 13 for analysis. |
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Dosage form |
0.1% in diet; oral administration; for 10 days. |
|
Applications |
Feeding a diet containing Sphingomyelins significantly lowered the disease activity index (DAI) score increased by DSS, prevented the increase in myeloperoxidase (MPO) activity in colonic tissue induced by DSS, and increased the amount of IgA in the contents of the large intestine. |
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References: |
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| Cas No. | 475662-40-9 | SDF | |
| 别名 | SMs (buttermilk) | ||
| Canonical SMILES | [R]C(N[C@@H](COP(OCC[N+](C)(C)C)([O-])=O)[C@H](O)/C=C/CCCCCCCCCCCCC)=O | ||
| 分子式 | C46H93N2O6P (for tricosanoyl) | 分子量 | 801.2 |
| 溶解度 | Chloroform: soluble,Ethanol: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2481 mL | 6.2406 mL | 12.4813 mL |
| 5 mM | 249.6 μL | 1.2481 mL | 2.4963 mL |
| 10 mM | 124.8 μL | 624.1 μL | 1.2481 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
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