SenTraGor™ cell senescence reagent is a detection reagent for lipofuscin, a nondegradable lysosomal marker of cellular senescence.1 It is composed of a Sudan Black B (SBB) derivative conjugated to biotin and can be detected using an anti-biotin antibody. SenTraGor™ cell senescence reagent has been used to detect lipofuscin accumulation in Vero E6 cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and primary non-small cell lung cancer (NSCLC) samples as well as soluble lipofuscin in serum from patients with various diseases, including dementia and rheumatoid arthritis.2,3,4
1.Evangelou, K., Lougiakis, N., Rizou, S.V., et al.Robust, universal biomarker assay to detect senescent cells in biological specimensAging Cell16(1)192-197(2017)
2.Evangelou, K., Veroutis, D., Paschalaki, K., et al.Pulmonary infection by SARS-CoV-2 induces senescence accompanied by an inflammatory phenotype in severe COVID-19: Possible implications for viral mutagenesisEur. Respir. J.2102951(2022)
3.Giatromanolaki, A., Kouroupi, M., Balaska, K., et al.A novel lipofuscin-detecting marker of senescence relates with hypoxia, dysregulated autophagy and with poor prognosis in non-small-cell-lung cancerIn Vivo34(6)3187-3193(2020)
4.Rizou, S.V., Evangelou, K., Myrianthopoulos, V., et al.A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in FluidsCellular senescence: Methods and protocols. Methods in molecular biology 1896119-138(2019)