Ribavirin

Cell lines

HeLa S3 cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

100 ~ 1,000 μM

Applications

HeLa S3 cells pre-treated with 100 μM Ribavirin before the infection with poliovirus at a low multiplicity of infection exhibited a reduction in virus production to 50%. Pre-treatment with 1,000 μM Ribavirin induced a reduction in virus production to 0.00001%.

Animal models

H1N1 virus-infected mice

Dosage form

In H1N1 virus-infected mice, Ribavirin in combination with Glycyrrhizin significantly inhibited the lung consolidation by 36%. The combination also exhibited synergetic effect on survival of infected mice. In addition, the combination of Ribavirin and Glycyrrhizin substantially reduced lung virus titer, as well as inhibited H1N1 virus-induced production of proinflammatory cytokines IL-6, TNF-α and IL-1β.

Applications

40 mg/kg/day

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Crotty S, Maag D, Arnold JJ, Zhong W, Lau JY, Hong Z, Andino R, Cameron CE. The broad-spectrum antiviral ribonucleoside ribavirin is an RNA virus mutagen. Nat Med. 2000 Dec;6(12):1375-9.

[2]. Chen XX, Zhou HX, Qi WB, Ning ZY, Ma YJ, Li YL, Wang GC, Chen JX. [Antiviral effects of the combination of glycyrrhizin and ribavirin against influenza A H1N1 virus infection in vivo]. Yao Xue Xue Bao. 2015 Aug;50(8):966-72.