Rhodamine 6G hydrazide (R6GH) is a fluorescent dye. Rhodamine 6G hydrazide can be used in selective colorimetric and fluorescent sensing.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Rhodamine 6G hydrazide (1.0 × 10 3 M) is prepared in methanol.
2. Dilute sample and Rhodamine 6G hydrazide to the working concentration.
3. Fill Rhodamine 6G hydrazide (2 mL, MeOH, 5 × 10 4 M) into a quartz cuvette, and then adds same amount of sample solution.
4. Record spectral data (Ex=500 nm, Em=505-100 nm).
References:
[1]. Upadhyay Y, et al. Optical sensing of hydrogen sulphate using rhodamine 6G hydrazide from aqueous medium. Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jun 5;180:44-50.