Rhodamine 6G (R6 G) is a lipophilic fluorescent cationic dye mainly used to detect the mitochondrial membrane potential (Δψ m) of cultured cells and isolated mitochondria[1]. Rhodamine dye is a cell-permeable fluorescent probe that specifically recognizes mitochondrial membrane potential and thus accumulates in mitochondria. Rhodamine dye has low toxicity to cells at a certain concentration, so it is often used to detect mitochondria in animal cells, plant cells, and microorganisms[2].
References:
[1]. Said H Audi,et. Quantification of mitochondrial membrane potential in the isolated rat lung using rhodamine 6G. 2020 Apr 1;128(4):892-906. doi: 10.1152/japplphysiol.00789.2019. Epub 2020 Mar 5.
[2]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.
Rhodamine 6G（R6 G）是一种亲脂性荧光阳离子染料，主要用于检测培养的细胞和分离的线粒体的线粒体膜电位（Δψ m ）[1]。Rhodamine染料是一种细胞渗透性荧光探针，特异性识别线粒体膜电位，从而在线粒体中积累。在一定浓度下，罗丹明染料对细胞的毒性较低，因此常用于检测动物细胞、植物细胞和微生物中的线粒体[2]。