Real Thiol (RT) is a reversible reaction-based fluorescent probe that can quantitatively monitor the real-time glutathione (GSH) dynamics in living cells. Real Thiol and its GSH adduct (RT-GSH) shows fluorescence maxima at 487 and 562nm with excitation wavelengths at 405 and 488nm, respectively. The binding rate constant (Kon) of the reaction between Real Thiol and GSH is 7.5M−1s−1, the dissociation constant (Koff) is 0.0203s−1, and the equilibrium dissociation constant (Kd) is 3.7mM. Real Thiol exhibits a wide dynamic range of proportional fluorescence response when reacting with glutathione, enabling the monitoring of GSH level changes in live cells within the 1-10mM range. Real Thiol is a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells[1].
References:
[1] Jiang X, Chen J, Bajić A, et al. Quantitative real-time imaging of glutathione. Nat Commun. 2017;8:16087.
Real Thiol（RT）是一种基于可逆反应的荧光探针，可以定量实时监测活细胞中的谷胱甘肽（GSH）动态。Real Thiol及其GSH加合物（RT-GSH）分别在487和562nm处显示最大荧光，其激发波长分别为405和488nm。Real Thiol与GSH反应的结合速率常数（Kon）为7.5M−1s-1，解离常数（Koff）为0.0203s−1，平衡解离常数（Kd）为3.7mM。Real Thiol与谷胱甘肽反应时显示出具有宽动态范围的比例荧光响应，可在活细胞中监测1-10mM范围内的GSH水平变化。Real Thiol是一种多功能工具，可用于共聚焦显微镜和基于流式细胞术的单细胞中谷胱甘肽水平的高通量定量[1]。