Quercetin D5 is a deuterated internal standard for the natural compound quercetin, its chemical structure is essentially the same as quercetin, except that five deuterium (D) atoms replace five hydrogen (H) atoms in the A ring[1]. By monitoring the specific mass spectral ion pairs of Quercetin D5 and natural quercetin and calculating their response ratios, a highly precise and accurate standard curve can be constructed, enabling the absolute quantification of extremely low concentrations (nanogram/mL level) of quercetin in biological samples[2, 3]. Using Quercetin D5 as an internal standard allows for the accurate determination of quercetin concentrations in the plasma and tissues of rats after oral administration, and the calculation of reliable pharmacokinetic parameters[4]. Quercetin is a natural flavonoid compound with applications in various research areas, including anti-inflammatory, anti-allergic, antiviral, cardiovascular protective, and anti-cancer activities[5].
References:
[1] Rasku S, Wähälä K. Synthesis of deuterium labeled polyhydroxy flavones and 3-flavonols[J]. Tetrahedron, 2000, 56(6): 913-916.
[2] Ma L, Peng Y, Cao D, et al. Difference Analysis of Non-Volatile and Volatile Components in Kujingcha (Ilex dabieshanensis) Compared with Green Tea (Camellia sinensis)[J]. Horticulturae, 2025, 11(7): 804.
[3] Wolfender J L. HPLC in natural product analysis: the detection issue[J]. Planta medica, 2009, 75(07): 719-734.
[4] Pilařová V, Plachká K, Chrenková L, et al. Simultaneous determination of quercetin and its metabolites in rat plasma by using ultra-high performance liquid chromatography tandem mass spectrometry[J]. Talanta, 2018, 185: 71-79.
[5] Aghababaei F, Hadidi M. Recent advances in potential health benefits of quercetin[J]. Pharmaceuticals, 2023, 16(7): 1020.
Quercetin D5是天然产物槲皮素（Quercetin）的氘代同位素内标，其化学结构通常在槲皮素的A环上由5个D原子取代了原有的5个H原子[1]。通过监测Quercetin D5与天然槲皮素特定的质谱离子对，并计算其响应比值，可以构建高精密度和准确度的标准曲线，实现对生物样本中极低浓度（纳克/毫升级别）槲皮素的绝对定量[2, 3]。使用Quercetin D5作为内标，能够精确测定大鼠口服槲皮素后的血浆和组织浓度，并计算可靠的药动学参数[4]。Quercetin是一种天然黄酮类化合物，能够用于抗炎、抗过敏、抗病毒、心血管保护以及抗癌等多种研究领域[5]。