Pseudo-UTP is derived from UTP through base isomerization reaction, which can perform complementary base pairing with adenosine like uridine, and change the structure of RNA by improving base pairing, base stacking and main chain stability [1]. The mRNA in which Pseudo-UTP completely replaces UTP exhibits lower immunogenicity, higher stability and translation ability [2].
References:
[1]. Yi-Tao Yu. RNA-guided isomerization of uridine to pseudouridine-pseudouridylation. Clin Chim Acta. 2014;11(12):1483-94. doi: 10.4161/15476286.2014.972855. PMCID: PMC4615163.
[2]. Muthian Shanmugasundaram, et al. An Efficient Protection-Free One-Pot Chemical Synthesis of Modified Nucleoside-5'-Triphosphates. Nucleosides Nucleotides Nucleic Acids. 2016 Jul 2;35(7):356-62.
Pseudo-UTP是由UTP通过碱基异构化反应衍生而来,可以像UTP一样与腺苷酸进行碱基互补配对,通过改善碱基配对、碱基堆叠和主链稳定性来改变RNA结构[1]。Pseudo-UTP完全替代UTP的mRNA表现出更低的免疫原性,更高的稳定性以及翻译能力[2]。