PRRSV/CD163-IN-1 is a PRRSV/CD163 inhibitor. PRRSV/CD163-IN-1 can inhibit the interaction between the PRRSV glycoprotein (GP2a or GP4) and the CD163-SRCR5 domain. PRRSV/CD163-IN-1 can be used for the research of porcine reproductive and respiratory syndrome (PRRS) [1].
PRRSV/CD163-IN-1 (Compound B7) (5 μM) 抑制 PRRSV GP4 糖蛋白和 CD163-SRCR5 结构域之间的蛋白间相互作用 (PPI)[1]。
PRRSV/CD163-IN-1 (24 h) 在浓度低于 25μM 时可被 PAMs 良好耐受，LC50 值为 81.7 μM[1]。< br/>PRRSV/CD163-IN-1 (0, 5, 10, 15, or 20 μM; 4 h) 以剂量依赖性方式抑制 PRRSV 感染 PAMs[1]。
PRRSV/CD163-IN-1 (15 μM) 显着抑制由 I 型和 II 型 PRRSV 菌株引起的感染[1]。
RT-PCR[1]
| Cell Line: | Porcine alveolar macrophages (PAMs) |
| Concentration: | 0, 5, 10, 15, or 20 μM |
| Incubation Time: | 4 h |
| Result: | Revealed a dose-dependent inhibition of PRRSV infection of PAMs. |
[1]. Chang Huang, et al. Small molecules block the interaction between porcine reproductive and respiratory syndrome virus and CD163 receptor and the infection of pig cells. Virol J. 2020 Jul 30;17(1):116