Protease Inhibitor Cocktail (100X in DMSO, EDTA plus)

Protease Inhibitor Cocktail (100X in DMSO, EDTA plus), which is used during cell lysis and protein extraction, is a ready-to-use concentrated stock solutions of multiple protease inhibitors for endogenous protease. This product contains a separate 0.5M EDTA solution which inhibiting metalloproteases.

It generates optimized concentrations of six broad-spectrum protease inhibitors stabilized in DMSO, that powerfully inhibits serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases appearing in cellular lysate samples. EDTA provides inhibition for metalloproteases.

EDTA inhibits metalloproteases by chelating the divalent cations that is necessary for their activity, so the activities of other proteins may be affected by EDTA. Therefore, empirical testing may be need in particular experiments to determine if EDTA will take a bad impact. If the protein of interest is to be purified using immobilized metal chelate affinity chromatography (IMAC) or analyzed by 2D gel electrophoresis, EDTA must be removed by extensive dialysis or desalting before purifying.

蛋白酶抑制剂混合物（100倍浓缩于DMSO中，含EDTA），用于细胞裂解和蛋白质提取，在其中添加了多种内源性蛋白酶抑制剂。该产品包含单独的0.5M EDTA溶液，可抑制金属蛋白酶。

该产品混合了六种广谱蛋白酶抑制剂，以DMSO为载体，具有强效抑制细胞裂解物中的丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬酰胺酸蛋白酶和氨肽酶等蛋白酶的功能。EDTA提供对金属蛋白酶的抑制作用。

EDTA通过螯合双价阳离子来抑制金属蛋白酶的活性，因此其他蛋白质的活性可能会受到影响。因此，在特定实验中可能需要进行经验性测试，以确定EDTA是否会对实验产生不良影响。如果要使用固定化金属螯合亲和层析（IMAC）纯化感兴趣的蛋白质或通过二维凝胶电泳分析蛋白质，则必须在纯化之前通过广泛的透析或脱盐去除EDTA。