The core principle of ECL reagent detection is the luminescence of the oxidation reaction: Luminol (lumino) is the main component of the luminescence substrate. Under alkaline conditions, it is catalyzed by H2O2 to generate 3-amino ozone. The excited state intermediate of phthalic acid emits photons when it returns to the ground state. The maximum emission wavelength is 425 nm. The photon signal can be captured by X-ray film or CCD imager.
The GlpBio Ultra High Sensitivity ECL kit can oxidize luminol in the presence of HRP and peroxide to detect within the femtogram range of antigens. This reaction produces prolonged chemiluminescence, which can be seen on X-ray film or digital imaging systems. The GlpBio Ultra High Sensitivity ECL kit can produce a powerful, long-lived signal. Coupled with a very low background level, it can extend the exposure time to detect low-abundance proteins.
ECL试剂检测的核心原理是氧化反应的发光：Luminol（lumino）是发光底物的主要成分。在碱性条件下，它被H2O2催化生成3-氨基臭氧。邻苯二甲酸激发态中间体返回基态时会释放出光子。最大发射波长为425纳米。光子信号可以被X射线胶片或CCD图像仪捕获。
GlpBio超高灵敏度ECL试剂盒可以在HRP和过氧化物存在的情况下氧化发光素，检测到飞克级别的抗原。这个反应产生持久的化学发光，在X射线胶片或数字成像系统上可见。GlpBio超高灵敏度ECL试剂盒可以产生强大、长寿命的信号。加上非常低的背景水平，它可以延长曝光时间以检测低丰度蛋白质。图示为ECL试剂盒原理图。